June 13, 2019

Do soy isoflavones help fight against PRRSV infection in weaning pigs & does soy source matter?

Tags: Swine | Nursery Pig | Immunity

Do soy isoflavones help fight against PRRSV infection in weaning pigs & does soy source matter?

Hello! Welcome to my Research Digest #2.

Today, the article I selected to share with you is titled 'Effects of dietary soy isoflavones and soy protein source on response of weaning pigs to porcine reproductive and respiratory syndrome viral infection'

This is a work from Dr. Ryan Dilger's lab at University of Illinois, and it just came out as an Advance article on the Journal of Animal Science, although I believe the study was conducted back in 2017.

Background

On one hand - Higher soybean meal (SBM) inclusion in pigs was shown to improve performance under immune challenges, which may be partly due to isoflavones (ISF, a class of bioactive components high in SBM), but previous work did not consider potential confounding factors like different amino acid levels or source. On the other hand - other previous work (mainly from Iowa State) suggested that ISF have antiviral activity specifically against PRRSV (Porcine reproductive and respiratory syndrome virus, one of the most prevalent diseases in swine), but these studies used individual  compounds of ISF like genestein or daidzein, not ISF at natural composition or concentration in SBM.

Therefore, combining these 2 background info, this study was conducted to see whether soy-derived ISF at industry-relevant level could help weaning piglets fight against PRRSV, and if there were any difference in its efficacy when soy sources differ (but digestible amino acids remain identical)?

What was done?

The experiment was essentially a 2x2+1 factorial arrangement, and below is an illustration of what was done...

Products used in this study include an soybean bean meal concentrate (Arcon AF from ADM),  an enzyme-treated SBM (HP300 from Hamlet Protein) and soy isoflavones (Novasoy from ADM).

What was found?

I thought I would always include a figure on growth performance (if available) for those who'd like to see the production impact first.

  • Growth Performance: PRRSV challenge was successful (and as expected, very bad for performance); In terms of soy source, ET-SBM was better than SBM concentrate; ISF did not make a difference statistically.
(Recreated based on Smith et al., 2019)



Other key findings

  • ETSBM + ISF had the highest PRRS viral concentrations on 3 & 6 DPI (the authors were expecting lower levels by ISF...the discrepancy might be due to different methods used, i.e. rt-PRC here, or different PRRSV strain used).
  • PRRSV-infection ↑ cytotoxic T-cells%, ISF (independent of soy source) ↑ helper T-cells% & thus increased helper: cytotoxic ratio (CD4:CD8 ratio) --> ISF seems to have beneficial effect on adaptive immune response during recovery period of disease challenge? need further evaluation throughout the grow-fin period.

(Recreated based on Smith et al., 2019)

Take home message

PRRSV is definitely bad!

ISF may help with recovery of PRRSV infection in weaning pigs by improving Helper : Cytotoxic T-cell ratio, but no beneficial effects found on performance.

Full article access 👇

Effects of dietary soy isoflavones and soy protein source on response of weanling pigs to porcine reproductive and respiratory syndrome viral infection

Reference

Smith, B.N., Morris, A., Oelschlager, M.L., Connor, J. and Dilger, R.N., 2019. Effects of dietary soy isoflavones and soy protein source on response of weanling pigs to porcine reproductive and respiratory syndrome viral infection. Journal of animal science.


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